Quality of Life Labs Integrative medicine.

Comparisons of GCP with shark cartilage (powder or liquid) and other isoflavone extracts for their angiogenesis effects.

Preparation for samples: Dissolved in PBS or in DMSO by 100mg/ml concentration, and diluted with PBS, the final DMSO concentration is less than 1%, 0.2ml of samples was added in the eggs. The liquid shark cartilage was added by original liquid. Each sample was added into 3 eggs.

Dissolved in	GCP	Powder shark cartilage	Liquid shark cartilage	Product A	Product B
PBS	++++ (6/6)	-- (0/6)		-- (0/6%)	-- (0/6)
DMSO	++++ (6/6)	-- (0/6)	++++ (6/6)	-- ~ ++++ (2/6)	-- ~ ++++ (4/6)

* : +++++~++++: Significantly effective; _: No effective;
* ( ) : percentage of effectiveness in six eggs

Dissolved in PBS

PBS	GCP	Powder Shark Cartilage	Product A	Product B
Dissolved in DMSO

PBS	GCP	Liquid Shark Cartilage	Product A	Product B

Fig. 15 Comparison of GCP dissolved in PBS or DMSO with other commercial products

Comparison of GCP with other commercial products on inhibition of vessels in tumor located areas

Method: Tumor-bearing mice model transplanted with S-180 sarcoma. GCP, Product A, Product B were dissolved in tap water by 10% and administrated, p.o. by original liquid of 0.35ml/day/ mouse for two weeks.

Result: The vessels in tumor located area clearly were found in untreated control. Vessels almost disappeared in GCP-treated mice while other commercial products and shark cartilage did not show such effects (Fig. 16).


Control	GCP	Liquid Shark Cartilage	Product A	Product B

Fig. 16 Comparison of GCP with other commercial products on inhibition of vessels in tumor located areas.

Comparison of GCP with other commercial products on inhibition of tumor growth

Method: Tumor-bearing mice model transplanted with S-180 sarcoma
Administration: GCP, Product A, Product B: Dissolved in tap water by 10% and administrated, p.o. by the dosage of 0.35ml /day/mouse. Period: Two weeks.

Observation: Tumor growth curve, tumor weight.
Results: GCP significantly inhibited the tumor growth of S-180, while shark cartilage, Product A or B did not show this inhibitory effect.

Fig. 17 Tumor growth curve of S-180 sarcoma after treatment with GCP and other commercial products.

Fig. 18 Tumor weight of S-180 sarcoma after treatment with GCP and other commercial products.